PLEASE READ FIRSTHow to protect yourself from breathing ozone
What are vaginal insufflations?
What equipment you need
How to put the equipment together and how to use it
Risks and how to avoid them


You perform vaginal insufflations by infusing an ozone/oxygen gas mix into the female reproductive system with the help of a catheter. They are best performed in a continuous fashion: one attaches a catheter to the ozone output hose and allows for the ozone/oxygen gas mix to flow in for the desired amount of time.



In order to do VIs you need:

oxygen + ozone generator + catheter


ozone vaginal insufflations direct diagram

Oxygen source: Either an oxygen tank or an oxygen concentrator can be used. Both the oxygen concentrator and oxygen tank have to allow for low oxygen flows. A maximum flow of 1/8 LPM is recommended. (1/32 is less than 1/16, which in turn is less than 1/8 LPM).

Oxygen concentrator: there are several possibilities. One can either use a low flow oxygen concentrator which allows for oxygen flows of maximum 1/8 LPM or one can use a regular oxygen concentrator (which usually allows for oxygen flows of 0.5 to 5 LPM) and attach an external low flow regulator.

Oxygen tank: either industrial or medical oxygen tanks should be equipped with pediatric regulators. Pediatric or low flow regulators allow for oxygen flows from 1/32, 1/16 to 1/8 LPM.

Ozone generator: It should be a generator which uses ozone resistant materials in the air chamber, can be fed oxygen from an oxygen concentrator or an oxygen tank, can reliably produce stable ozone concentrations at given oxygen flows, and does not exceed ozone concentrations of 80 ug/ml. For direct rectal insufflations it should be able to generate ozone concentrations between 20 and 40 ug/ml (gamma) at oxygen flows of 1/32 to 1/8 LPM.

Accessories: Catheters: most are made of PVC, some use silicone. Instead of a catheter you can also use a bag or a syringe. Or use SimplyO3’s kit for vaginal insufflations, see below.

Best catheter: one with a luer lock attachment. No adapter necessary in order to connect it to your ozone output tubing. A simple luer lock connector is all you need.

For those who prefer to do VI indirectly: use Promolife’s new 200 ml polycarbonate syringe. Fill it with the ozone/oxygen gas, attach it to the inserted catheter, and insert the gas by pressing on the plunger. Make sure you also get an adapter if you opt for slip-on catheters. If you decide to go with luer lock catheters, get some spare luer locks as well.

A silicone catheter for vaginal (or rectal) insufflations from Promolife.

The “standard” PVC catheter which can be used for vaginal (or rectal) insufflations, also from Promolife.

Don’t forget to get the adapter: this allows you to connect a slip-on catheter to the ozone output tubing.


Suggested are the following settings:
Oxygen flow: maximum 1/8 LPM
Ozone concentration: 20 to 40 gamma
Duration: Start with 5 min per day. Increase slowly until you reach 20 minutes.
Some professional ozone practitioners suggest continuous VI continuously for up to 2 hours.

For an overview of the different connectors and tubing, please go to How to connect ozone equipment.

Connect the oxygen source (either tank or concentrator) with the ozone generator inlet via tubing. Attach silicone tubing to the ozone generator “ozone out” outlet. Insert a catheter into the vagina until you feel resistance or around 2 inches deep (6 to 8 cm). Turn on the oxygen and set it at the given oxygen flow. Connect the “ozone out” silicone tubing with the catheter. Turn on the ozone generator and set it on the desired ozone concentration. Place a towel on your lap which will absorb the excess ozone. Additionally, either wear a mask or put a fan in front of you. Open a window and make sure there is enough ventilation in the room.



  • Gas embolism (I personally think that risk is vanishingly small. I could only find one case where it was suspected that a woman died due to a gas embolism after a certain sexual practice which according to the coroner had pushed air through an open vein in her uterus and caused an air embolism. As far as I know, there is no reported case where this has ever occurred during vaginal ozone insufflations. Still, Dr. Rowen prefers to make people aware of it.)
  • Itchy rash and burning sensation
  • If you suspect having a tear or a wound due to trauma or other causes in your reproductive system, do not attempt vaginal insufflations. Otherwise you’re running a risk of pushing air into an open vein which could cause a gas embolism.
  • reduce the ozone concentration, frequency, or duration of applications




  • open wound in the reproductive system
  • transplanted organ

None of the ozone generators presented on this website have been approved for medical purposes. Any application of the machines for other than water purification is at the sole risk of the user.

Legal Disclaimer

Information provided is for informational purposes only and is not a substitute for professional medical advice. No health claims for these products or diagnostic tools have been evaluated by the United States Food and Drug Administration (FDA), nor has the FDA nor any other medical authority approved these products to diagnose, cure, or prevent disease. Since every person is unique, we highly recommend you to consult with your licensed health care practitioner about the use of ozone products in your particular situation. Neither The Power of Ozone nor the manufacturers of these items are responsible for the misuse of this equipment. It is highly advised to receive professional council from a licensed doctor before using Ozone Therapy or any of the mentioned products or tests on yourself.


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  1. karina

    What’s the ideal position for administering VI? Sitting? Laying down?

    • paola d

      I prefer to sit and work on my computer while insufflating. Find the position most comfortable for you.

  2. Jane Laursen

    IS the catheter used the same as for RI’s?

    • paola d


  3. Lia

    for systemic ozone is there any information re: rectal vs vaginal insufflation? The info I can find on rectal is that is is 80-90% as effective as MAH. I have successfully completed surgery ( 1 positive lymph node)chemo and radiation for aggressive uterine CA and now want to increase immune system and weaken any possible remaining cancer cells.

    • paola d

      Hi Lia,
      there is conflicting information on rectal and vaginal insufflation.
      Some allege that RI are a systemic treatment, others doubt it.
      The Cubans are big on rectal insufflations and seem to successfully treat a whole range of conditions with it.
      Vaginal insufflations are poo-pood by some, and embraced by other ozone experts.
      From personal experience I know VI are a systemic treatment, most likely through the lymphatic system.
      If I had had treatments for uterine CA, I would most likely concentrate on VI and some form of systemic treatment like ozone IVs at a doctor’s or ozone saunas.

    • Lia

      Thank you Paola — just found this site again. Started VI and RI at home and have 2 10 pass scheduled starting the first of January. Then plan on doing a series of MAH. Also do some EI for about 2-4 minutes 3-4 times a week.

      Thanks again

  4. Diane

    Is it okay to put ozone up the vagina everyday?

    • paola d


  5. Shannon

    I had VI ozone today for the first time by a naturopath. I have neuro lyme. He used 3 syringes of ozone via a catheter.
    I was lying down for the procedure. When I sat up there was copious fluid on the bed. The doctor didn’t know what that it was. It was clear and had no odour. It definitely wasn’t urine. What was this fluid that drained out of me and why did it happen?

    • paola d

      I don’t know exactly what it was.
      VI can stimulate natural secretions, maybe that is what it was?

  6. Kimberly

    I have actually found a vaginal insufflation device designed specifically for this use. It’s a vet supply, but I found out that the unit was actually designed for human use. This way you don’t get excess ozone escaping into the room and potentially damaging your lungs

    • Doina

      Hi Kimberley, which is the product from this page that you are referring to? Interested in buying something different for VIs than a catheter!

  7. Elizabeth

    I started vaginal ozone yesterday and immediately felt clear headed and more energized. Today I am experiencing a lot of yellow discharge. No odor and no pain/itch etc. I feel fine but the discharge is alarming. Anyone else have this happen?

    • paola d

      Hi Elizabeth,
      the discharge happens often. It is possible it is just normal vaginal secretions which ozone is known to stimulate.

  8. Susana


    Did VI ever cause you start your menstruation? I know that I am not suppose to use ozone few days prior and after my menstruation so I normally wait 4-5 days after I’m done. Within two days of doing VI I start my period again and this makes it frustrating! just wanted to get your impute on this.

    • paola d

      Hi Susana,
      no, this never happened to me. But I have heard this a few times. So it seems it is possible.

  9. Kay

    Hi Paola, I had read that when doing longer VI treatments (2 hours), that the humidification bottle may be attached in order to add moisture & not dry out the vaginal tissue. You’ve been so helpful on everything ozone. Just wondering if you think it’s a good idea or not to add humidification to the VI treatment. Thank you.

    • paola d

      Hi Kay,
      yes with prolonged VI humidification might be a good idea.
      Just make sure you protect your ozone generator from water backflow.


  10. Natasha

    Hello Paola, I am wondering if VI can be as effective in delivering O3 to the blood stream as RI. I’ve been developing painful gas reactions to RI while VI run very smoothly for me, so I prefer to continue with them. It would seem to me as common sense, O3 would penetrate as well from rectal tissues as through vaginal walls? I’ve got a variety of viral conditions am currently treating with it. Thank you!

    • paola d

      Hi Natasha,
      contrary to what most ozone practitioners say, I do not think that RI are a good method to deliver ozone into the blood stream or that they are a good systemic treatment at all. With prolonged treatment one can achieve systemic effects in some conditions, but overall RI are not comparable to intravenous administrations, in my opinion.

      VI are in my opinion a better way to achieve systemic effects if done long enough and with high enough volumes. They are also easier to perform and less messy.

      Yes, I believe that with prolonged application, the O3 and its by-products is able to penetrate the intestinal walls and probably also the lymph.

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Hi! I’m Paola. I’ve been using ozone for over 11 years and I’m here to share with you what I have learned.

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