How To Do An Intramuscular Ozone Injection (with Pictures)

by | Last updated: Mar 1, 2024 | 42 comments

An intramuscular ozone injection is the injection of a mix of ozone and oxygen gas into a muscle. Either the dorsogluteal or the ventrogluteal muscle can be used. In this guide I will demonstrate the injection into the dorsogluteal area.  

Intramuscular ozone injections have been used by some doctors on Coronavirus patients with surprising results. So it looks like like this type of ozone treatment could have systemic effects.

During an intramuscular ozone injection a relatively low ozone concentration of around 10 to 20 mcg/ml and a low amount of 10 to 20 ml are applied.

What equipment do you need for an intramuscular ozone injection?

  • oxygen tank and regulator (Where can you get medical oxygen for ozone injections?)
  • ozone generator
  • ozone syringe
  • syringe filling station with a filter
  • needles
  • tank wrench
  • alcohol wipes
  • cotton pad
  • bandaid
  • gloves
  • sharps bin
  • a tall glass
  • silicone tubing to connect oxygen tank and ozone generator
  • mirror

Scroll down to find a list with the recommended equipment.

How to do an intramuscular ozone injection

Suggested settings

Ozone concentration: 20 to 40 mcg/ml
Gas volume: 10 to 20 ml


Suggested settings

Ozone concentration:

20 to 40 mcg/ml

Gas volume:

10 to 20 ml

1. Screw a low flow regulator onto the oxygen tank.

2. Take a piece of silicone tubing, and connect one end to the oxygen tank. 

3. Take the other end and push in a luer lock connector. 

4. Screw the end with the luer lock connector into the “oxygen in” port of the ozone generator.

5. Connect the ozone generator to the power supply. 

6. Screw the syringe filling station onto the “ozone out” port of the ozone generator. Make sure the OFF valve is turned towards the destructor. 

7. Open the valve of the tank, half a turn is enough. Use the wrench key for this. Watch the gauge jump up – it shows you how much oxygen you have left in your tank. 

8. Open the regulator to 1/2 LPM.

9. Count to 15 to let pure oxygen flow through the silicone tubing, the ozone generator, the syringe filling station, and the filter. You flush the lines with pure oxygen. 

10. Now flush the syringe: screw it onto the filling station, let it fill all the way with pure oxygen. 

11. Unscrew and empty it by pushing on the plunger. 

12. Check your ozone output chart for the right oxygen flow and ozone setting. For intramuscular ozone injections an ozone concentration between 10 and 25 mcg/ml can be used. I pick an ozone concentration of 15 mcg/ml, which means that the oxygen flow has to be set at 1/2 LPM and the first ozone dial on 5, the second ozone dial will be off. 

13. My oxygen regulator is already set at the right flow, 1/2 LPM. (The ozone syringes have a slower moving plunger, so it's better to use a higher oxygen flow.)

14. Close the syringe port on the syringe filling station by turning the OFF lever towards the filter. 

15. Screw the syringe onto the filter of the syringe filling station. 

16. Set the dial on your ozone generator to the desired setting. Here, it's 5 for the first dial, the second dial remains off. 

17. Open the syringe port on the syringe filling station by turning the OFF lever towards the destructor. Now, ozone/oxygen is flowing into the syringe. 

18. Fill the syringe with 25 cc of ozone/oxygen mix. Fill the syringe with 5 cc more ozone/oxygen than the amount you will inject. 

19. Once 25 cc have been reached, close the syringe port by turning the OFF lever towards the syringe port. 

20. Turn the ozone generator off. 

21. Close the oxygen regulator by setting it to zero. 

22. Unscrew the syringe, keep it upright to prevent ozone from pouring out and air from getting in. Ozone is heavier than air. 

23. Open the packaging of the needle. 

24. Screw the needle onto the syringe. 

25. Set it aside. Place it vertically in a glass, while you prepare the rest. 

26. Put gloves on.

27. Pick a spot where you will inject the ozone. Draw a horizontal line where your butt crack begins (that's where my finger is). Divide that line in half vertically. The upper outer quadrant is where you want to inject. This is the dorsogluteal muscle. 

28. Disinfect the injection site with an alcohol wipe. 

29. Remove the plastic cap from the needle. 

30. Push out 5 cc to flush the needle. 

31. Inject straight into the muscle, possibly at a 90 degrees angle (unlike what you see in the picture ). It helps to place yourself in front of a mirror.

32. Aspirate by pulling gently on the plunger. See if any blood comes out. If it does, it means you hit a blood vessel. In which case you need to pull the syringe out and inject in a different spot. If no blood is visible, you can continue with the injection. 

33. Inject the content of the syringe by pressing on the plunger. You may feel some pressure. 

34. When done, pull the needle out, and press down on the puncture wound with a cotton pad. 

35. Put a bandaid on.

36. Unscrew the needle carefully from the syringe and place it in a sharps bin.

37. When you're done using ozone for the day, then make sure to release the pressure from the regulator. To do this, first close the valve on the tank using a wrench. Do not exert too much force. 

38. Open the regulator all the way by setting it to the highest flow. Here, it's 4 LPM. You will hear a swishing sound. Watch the gauge go all the way down to zero. 

39. Set the regulator to zero. Good job, you're done!

Recommended equipment for an intramuscular ozone injection

This is the equipment I used in the pictures above:

What are intramuscular ozone injections used for?

Intramuscular ozone injections are used as a mild form of a systemic treatment. It is assumed that the beneficial ozone byproducts, once the ozone is absorbed by the muscle, enter the blood stream and can so circulate throughout the body. 

Dr. David Brownstein has used IM ozone injections to successfully treat Covid-19 patients together with other interventions. 

Risks of and contraindications for intramuscular ozone injections

Risks / side effects:

  • gas embolism, if the injection is accidentally performed into a blood vessel
  • nerve damage, if the ozone is accidentally injected into a nerve
  • local hematoma (bruise)
  • localized pain, or sensation of pressure after the injection


  • Transplanted organ, since ozone could overstimulate the immune system and trigger a rejection of the foreign organ.

Success stories with intramuscular ozone injections

Dr. David Brownstein documented 37 cases of patients who either tested positive for Covid-19 or were assumed to be sick with the virus and who received intramuscular ozone injections in combination with other alternative treatments. All 37 patients recovered. 

The Crazy Ozone Lady's take on intramuscular ozone injections

For the purpose of this guide I have performed a few of the IM ozone injections, and my verdict: I'm not a fan. 

There is some pain involved in the procedure and it's not easy to do since some twisting is required. 

Also, in spite of Dr. Brownstein's remarkable success, I remain skeptical about their assumed systemic effect. It's important to note that Dr. Brownstein was using the ozone injections on Covid-19 patients always in combination with other potent therapies. 

All in all, if I am in need of a systemic ozone treatment, I prefer DIV ozone injections. 

Be prepared for the next pandemic and learn how to use ozone therapy in the most effective way. Pick the Crazy Ozone Lady's brain and book a call with her.

About the author:

PaolaI’m Paola the Crazy Old Ozone Lady behind The Power of Ozone. I’m a licensed naturopathic practitioner, natural health consultant, ozone therapy enthusiast, researcher, and ozone therapy analyst. I hold certificates in ozone therapy, hyperbaric ozone applications, Oxyvenierung, and the Andrew Cutler chelation. I own several ozone generators including a German hyperbaric 10 Pass machine. I have been using ozone for over 13 years, I’ve chelated with the ACC program for close to 5 years and I’ve been carnivore for nearly 1.5 years. This website serves as a resource for those who are interested in ozone therapy and other approaches to successfully manage chronic conditions.

Legal Disclaimer

Information provided is for informational purposes only and is not a substitute for professional medical advice. No health claims for these products or treatments have been evaluated by the United States Food and Drug Administration (FDA), nor has the FDA nor any other medical authority approved these treatments or products to diagnose, cure, or prevent disease. Neither The Power of Ozone nor the manufacturers of these items are responsible for the misuse of this equipment or the information on this page. It is highly advised to receive professional council from a licensed doctor before using ozone therapy or any of the mentioned products or tests on yourself.

Affiliate Disclosure

This website contains links to vendors of products I endorse, including If you decide to make a purchase through one of my links, they will pay me a commission. This does not influence my opinion about the products, as you can see from my reviews.


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  1. Christa

    The needle link is not active.

    Do you have another source for purchasing needles?

  2. Juan

    Will ozone injection while recovering from hip replacement be contraindicated? I want to speed up recovery.

    • Paola Dziwetzki

      Hi Juan,

      depends where the injections would be placed and what type of injections? If you are referring to intravenous ozone, then they could potentially help. But I would wait for some time until there is some minimum of healing and that the anti-coagulants that are typically used in ozone IVs don't cause some bleeding from the wound.

      Maybe rinsing with ozonated water could help.


  3. A

    What if I don't have the dual ozone machine and only the single one, what would the measurements be?

    • Paola Dziwetzki

      Hi A,

      I would suggest the same settings: 1/2 LPM and dial on 5. According to the ozone output chart this should give 14 mcg/ml.


  4. Sheatina Sparks

    Hello. I’m suffering from all over body and joint pain and PGAD and chronic debilitating bladder pain. I want to learn how to use ozone for bladder instillations and also muscle and joint injections. Where are you located? I’d like the 10 pass system to help my whole body. I’ve had injections before and it was like a miracle. Saved me from surgery twice. Where do I start? Thank you

    • Paola Dziwetzki

      Hi Sheatina,

      Are you interested in buying a 10 pass machine? Or a simpler setup to do bladder insufflations and muscle and joint injections?

      I would need to understand better what you are interested in in order to suggest the right equipment for you.

      The 10 pass treatment calls for a hyperbaric ozone machine that is only sold to licensed professionals. You also need heparin (an anti-coagulant which is prescription medication) and ideally a nurse to help you administer it. A 10 pass machine costs close to Euro 12000 plus VAT plus customs fees plus supplies.

      For bladder insufflations, muscle and joint injections you can use simpler ozone generators that run for $800 to $1,800 plus oxygen tank and other supplies.

      If you want to know more, feel free to schedule a call with me:


  5. Minxie

    Is there an amount of ozone that would be unsafe to inject? How many shots are safe to do at once in the stomach/back area? And how much would be a danger? (Would 20 be a lot?)

    • Paola Dziwetzki

      Hi Minxie,

      It sounds like you're asking about subcutaneous, not intramuscular injections?

      If so, there is no limit how much you can inject, as far as I know.

      When doing subcutaneous injections the ozone amount is usually 2 to 5 ml per puncture site. And depending on how many punctures you do, it can be 20 ml or more …


  6. Vestrand Kim

    Do you have any success stories in shrinking cysts with ozone? Specifically tarlov cysts? If not, do you know any alternative healing treatments for them, to shrink or reduce and relieve pain? Thank you so much in advance.

    • Paola Dziwetzki

      Hi Kim,

      I'm afraid, I don't know much in regards to Tarlov cysts. It's possible ozone would work, but that no one has tried it yet …

      This may be a long shot, I don't know: but there is some speculation that the body produces all kinds of cysts due to oxalates. So maybe reduce oxalate rich foods, and maybe this will help shrink it.

      Maybe ozone saunas could help as well?


    • Kim

      Paula, I truly appreciate the response. Do you know if there is any chance that ozone could make it worse?

    • Paola Dziwetzki

      Hi Kim,

      Maybe if the doctor is not working cleanly enough, then there may be a risk of infection.

      Other than that – I don't think so…

  7. kaine

    Hi! I chaffed my armpits longtime ago (means i was harsh at shaving them quickly without using soap or anything) the skin got red hurt but i took iboprofin then the skin was good.
    But since then my armpits always smell very bad even if i use aluminum it works just 4 hours or so , before that i only use natural deodorant and my armpits still smell good the next day. I tried all natural things henna,gentian violet … ABSOLUTLY NOTHING WORKS!

    The only way i found to get the smell out is ozone 1/32 or 1/16 during 15 min or more but i need to do it everyday on each armpit.
    After seeing your video i want to do it intradermal , i want to buy the needle that you mention here but i dont know which size ( it s skin am targeting not the muscle). so i have few questions please,
    if i want to ozonated my skin armpits.

    1-should i buy the same size of needle or smaller for the skin injection?
    2-how deep should the needle be inserted ?
    3-how much concentration and volume ?
    4-how much cm square of the surface area one injection will cover?
    5-finally how often?

    • Paola Dziwetzki

      Hi kaine,

      sounds like what you're attempting could be extremely painful.

      1-I'd look for 30g 13 mm needles.
      2-if you're going intradermal, you're injecting just the tip of the needle, so very shallow injection. You want to create a gas bubble right under the upper skin layer.
      3-maybe 10 to 20 mcg/ml? I'm not sure you will be able to inject much at all, so maybe 1 to 2 ml.
      4-depends on how much you'll be able to inject, but I doubt it will be much. But try it out and report back.
      5-no idea. I don't know that what you're proposing here has ever been done before, or if it was successful. You're in uncharted territory as far as I know.

      Good luck.

  8. Magnus

    What do you think about doing this not as a systemic treatment, but to locally treat muscle pain? I am an athlete with low back pain and I am considering IM ozone injections into the lumbar erectors.

    • Paola Dziwetzki

      Hi Magnus,

      yes, you can definitely do that, but use a 30 g, 1/2 inch needle for that.

      Ozone saunas could also help a great deal.


    • Magnus

      Ok great,
      I tried ozone sauna first and ozone oil. It seems to help so maybe that is enough to get me through training.

  9. Kathi Bomersbach

    ok… Thank you for that information.

    I have so much to learn, and am just entering into this.

    Can I contact you for a consultation?

    • Paola Dziwetzki

      Hi Kathi,

      yes of course.
      I just sent you an email which explains how to go about it.
      Talk to you soon!


  10. Kathi Bomersbach

    Hi Paola,
    I have been researching personal ozone use units for quite awhile now, and I am really thankful to find your site. I'm ready to finally pull the trigger and purchase one, and your insight was certainly helpful.
    Thank you for sharing so much information, and for your willingness to be a guinea pig for us.
    I have a couple medical devices implanted (spinal cord pain pump & neurostimulator), and I have obviously been worried about what can grow on the implants… and it contributing to my RA & PsA by being another Autoimmune agitation.
    Do you feel like IM injections of ozone would help remove any biofilms or fungus's that might attach to a medical device?

    • Paola Dziwetzki

      Hi Kathi,

      I doubt that IM injections work in that way.

      But they could help you with any possible autoimmune issues.

      I would rather suggest ozone saunas or ozone body bags though.


  11. Deidre Bridges

    You are simply the best. I have learned so much from you and can't wait to try this. Thank you for sharing so much with us so that we can all experience better health! God bless you, Paola!

    • Paola Dziwetzki

      Hi Deirdre,

      thank you! I appreciate you, too!


  12. Jimmy

    I always enjoy reading your articles. There always full of interesting and useful information. Thank you for posting and sharing this.

    • Paola Dziwetzki

      Hi Jimmy,

      thank you!

  13. Phill B

    Thanks for the info. Can the same procedure be used direct into ankles and knees?

    • Paola Dziwetzki

      Hi Phill,

      with a smaller needle and a slightly different technique, yes.


  14. Joy

    Hello! How often do you think you can perform this therapy? How often should you switch up sides? Thank you!

    • Paola Dziwetzki

      Hi Joy,

      I would switch up sides every time.
      I think as long as there is bruising or pain or any other irritation, you can do it indefinitely.
      You can also use your thigh muscles.


    • Joy

      Thank you! What is the thigh muscle you can inject into? I have to YouTube it!

  15. Jacquelyn Fellows

    Paola you are the best! I started IM's before you posted these directions and I was winging it. I definitely learned from your tutorial..thank you for being so clear about the procedure. The current flu economy has me so strapped but in a few months when I recover I am looking forward to a private session with you.

    • Paola Dziwetzki

      Hi Jacqui,

      happy to hear that it was helpful!

      Whenever you're ready – you know where to find me :-). And thank you for your earlier donation!


  16. victoria

    Thank you so much, Paola, for this awesome tutorial!
    You must be an angel b/c you have answered my prayer.
    Taking a risk of embarrassment, despite reading this perfectly perfect, well-detailed instructions, I just have to ask:
    The syringe should be filled with 15mcg/ml of ozone without adding in any extra medical oxygen, correct?
    About 3 months ago, I visited a holistic practitioner to do ozone-through-oil insufflation. She said that ozone should not be injected undiluted. She even showed me how ozone dissolved the glove on her fingers as she was setting up the generator for the insufflation procedure. I had IV ozone injections more than 15 years ago, I don`t remember exactly if it was diluted. Cut the story short, I have purchased my own promolife generator (Thanks to your amazing blog), and I am a big fan of Dr Rowens and Dr Browstein. Now I am eager to do intramuscular ozone injections, just need to make sure I don`t burn a hole in butt (one is enough). Thank you, Paola!

    • Paola Dziwetzki

      Hi Victoria,

      I'm glad you took that risk, it allows me to engage in some smart-assery ;-).

      So, allow me to explain:

      When you use the highest possible ozone concentration which is allowed in ozone therapy, which is commonly accepted as being 80 mcg/ml, and if you filled a syringe with this, then the syringe would contain around 95% oxygen and only 5% would be ozone.

      If you use lower ozone concentrations, then this number falls even more.

      At an ozone concentration of 15 mcg/ml you'd be using around 1 to 2% ozone and the rest, so 98 to 99% would be oxygen.

      So when using ozone therapy, you ALWAYS use a dilution.

      Using 100% ozone – this is impossible with any of the common home ozone generators, and even less so with professional medical machines.

      The dissolution of the latex glove – this will happen at ANY ozone concentration, even the lowest one.

      I hope this helps.


    • victoria

      I am deeply thankful to you, Paola!
      You are awesome!

    • Paola Dziwetzki

      Hi Victoria,

      thank you very much!


  17. Caroline

    Thank you where do I get the needles can I inject In a different area's

    • Paola Dziwetzki

      Hi Caroline,

      there is a link to amazon under the “Recommended equipment” section at the end of the article.


    • Paola Dziwetzki

      You can also inject into the ventrogluteal area, which requires a bit less twisting.

      Possibly also into the thigh.


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