How To Do An Intramuscular Ozone Injection (with Pictures)

by | Apr 30, 2020 | 18 comments

An intramuscular ozone injection is the injection of a mix of ozone and oxygen gas into a muscle. Either the dorsogluteal or the ventrogluteal muscle can be used. In this guide I will demonstrate the injection into the dorsogluteal area.  

Intramuscular ozone injections have been used by some doctors on Coronavirus patients with surprising results. So it looks like like this type of ozone treatment could have systemic effects.

During an intramuscular ozone injection a relatively low ozone concentration of around 10 to 20 mcg/ml and a low amount of 10 to 20 ml are applied.

What equipment do you need for an intramuscular ozone injection?

  • oxygen tank and regulator (Where can you get medical oxygen for ozone injections?)
  • ozone generator
  • ozone syringe
  • syringe filling station with a filter
  • needles
  • tank wrench
  • alcohol wipes
  • cotton pad
  • bandaid
  • gloves
  • sharps bin
  • a tall glass
  • silicone tubing to connect oxygen tank and ozone generator
  • mirror

Scroll down to find a list with the recommended equipment.

How to do an intramuscular ozone injection


1. Screw a low flow regulator onto the oxygen tank.

2. Take a piece of silicone tubing, and connect one end to the oxygen tank. 

3. Take the other end and push in a luer lock connector. 

4. Screw the end with the luer lock connector into the “oxygen in” port of the ozone generator.

5. Connect the ozone generator to the power supply. 

6. Screw the syringe filling station onto the “ozone out” port of the ozone generator. Make sure the OFF valve is turned towards the destructor. 

7. Open the valve of the tank, half a turn is enough. Use the wrench key for this. Watch the gauge jump up – it shows you how much oxygen you have left in your tank. 

8. Open the regulator to 1/2 LPM.

9. Count to 15 to let pure oxygen flow through the silicone tubing, the ozone generator, the syringe filling station, and the filter. You flush the lines with pure oxygen. 

10. Now flush the syringe: screw it onto the filling station, let it fill all the way with pure oxygen. 

11. Unscrew and empty it by pushing on the plunger. 

12. Check your ozone output chart for the right oxygen flow and ozone setting. For intramuscular ozone injections an ozone concentration between 10 and 25 mcg/ml can be used. I pick an ozone concentration of 15 mcg/ml, which means that the oxygen flow has to be set at 1/2 LPM and the first ozone dial on 5, the second ozone dial will be off. 

13. My oxygen regulator is already set at the right flow, 1/2 LPM. (The ozone syringes have a slower moving plunger, so it’s better to use a higher oxygen flow.)

14. Close the syringe port on the syringe filling station by turning the OFF lever towards the filter. 

15. Screw the syringe onto the filter of the syringe filling station. 

16. Set the dial on your ozone generator to the desired setting. Here, it’s 5 for the first dial, the second dial remains off. 

17. Open the syringe port on the syringe filling station by turning the OFF lever towards the destructor. Now, ozone/oxygen is flowing into the syringe. 

18. Fill the syringe with 25 cc of ozone/oxygen mix. Fill the syringe with 5 cc more ozone/oxygen than the amount you will inject. 

19. Once 25 cc have been reached, close the syringe port by turning the OFF lever towards the syringe port. 

20. Turn the ozone generator off. 

21. Close the oxygen regulator by setting it to zero. 

22. Unscrew the syringe, keep it upright to prevent ozone from pouring out and air from getting in. Ozone is heavier than air. 

23. Open the packaging of the needle. 

24. Screw the needle onto the syringe. 

25. Set it aside. Place it vertically in a glass, while you prepare the rest. 

26. Put gloves on.

27. Pick a spot where you will inject the ozone. Draw a horizontal line where your butt crack begins (that’s where my finger is). Divide that line in half vertically. The upper outer quadrant is where you want to inject. This is the dorsogluteal muscle. 

28. Disinfect the injection site with an alcohol wipe. 

29. Remove the plastic cap from the needle. 

30. Push out 5 cc to flush the needle. 

31. Inject straight into the muscle, possibly at a 90 degrees angle (unlike what you see in the picture ). It helps to place yourself in front of a mirror.

32. Aspirate by pulling gently on the plunger. See if any blood comes out. If it does, it means you hit a blood vessel. In which case you need to pull the syringe out and inject in a different spot. If no blood is visible, you can continue with the injection. 

33. Inject the content of the syringe by pressing on the plunger. You may feel some pressure. 

34. When done, pull the needle out, and press down on the puncture wound with a cotton pad. 

35. Put a bandaid on.

36. Unscrew the needle carefully from the syringe and place it in a sharps bin.

37. When you’re done using ozone for the day, then make sure to release the pressure from the regulator. To do this, first close the valve on the tank using a wrench. Do not exert too much force. 

38. Open the regulator all the way by setting it to the highest flow. Here, it’s 4 LPM. You will hear a swishing sound. Watch the gauge go all the way down to zero. 

39. Set the regulator to zero. Good job, you’re done!

Recommended equipment for an intramuscular ozone injection

This is the equipment I used in the pictures above:

Be prepared for the next pandemic and learn how to use ozone therapy in the most effective way. Pick the Crazy Ozone Lady’s brain and book a call with her.

Legal Disclaimer

Information provided is for informational purposes only and is not a substitute for professional medical advice. No health claims for these products or treatments have been evaluated by the United States Food and Drug Administration (FDA), nor has the FDA nor any other medical authority approved these treatments or products to diagnose, cure, or prevent disease. Neither The Power of Ozone nor the manufacturers of these items are responsible for the misuse of this equipment or the information on this page. It is highly advised to receive professional council from a licensed doctor before using ozone therapy or any of the mentioned products or tests on yourself.

About the author:

PaolaI’m Paola the Crazy Old Ozone Lady behind The Power of Ozone. I’m a licensed naturopathic practitioner, natural health consultant, ozone therapy enthusiast, researcher, and ozone therapy analyst. I hold certificates in ozone therapy, hyperbaric ozone applications, Oxyvenierung, and the Andrew Cutler chelation. I own several ozone generators including a German hyperbaric 10 Pass machine. I have been using ozone for over 13 years, I’ve chelated with the ACC program for close to 5 years and I’ve been carnivore for nearly 1.5 years. This website serves as a resource for those who are interested in ozone therapy and other approaches to successfully manage chronic conditions.

Paola Dziwetzki, NP

I’m Paola, aka the Crazy Old Ozone Lady. I help people use ozone therapy in the most effective way. I have used ozone and diet to successfully treat psoriasis, joint pain, chronic fatigue, a low immune system, herpes infections, and a few other conditions.
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This website contains links to vendors of products I endorse, including If you decide to make a purchase through one of my links, they will pay me a commission. This does not influence my opinion about the products, as you can see from my reviews.


  1. Caroline

    Thank you where do I get the needles can I inject In a different area’s

    • Paola Dziwetzki

      Hi Caroline,

      there is a link to amazon under the “Recommended equipment” section at the end of the article.


    • Paola Dziwetzki

      You can also inject into the ventrogluteal area, which requires a bit less twisting.

      Possibly also into the thigh.


  2. victoria

    Thank you so much, Paola, for this awesome tutorial!
    You must be an angel b/c you have answered my prayer.
    Taking a risk of embarrassment, despite reading this perfectly perfect, well-detailed instructions, I just have to ask:
    The syringe should be filled with 15mcg/ml of ozone without adding in any extra medical oxygen, correct?
    About 3 months ago, I visited a holistic practitioner to do ozone-through-oil insufflation. She said that ozone should not be injected undiluted. She even showed me how ozone dissolved the glove on her fingers as she was setting up the generator for the insufflation procedure. I had IV ozone injections more than 15 years ago, I don`t remember exactly if it was diluted. Cut the story short, I have purchased my own promolife generator (Thanks to your amazing blog), and I am a big fan of Dr Rowens and Dr Browstein. Now I am eager to do intramuscular ozone injections, just need to make sure I don`t burn a hole in butt (one is enough). Thank you, Paola!

    • Paola Dziwetzki

      Hi Victoria,

      I’m glad you took that risk, it allows me to engage in some smart-assery ;-).

      So, allow me to explain:

      When you use the highest possible ozone concentration which is allowed in ozone therapy, which is commonly accepted as being 80 mcg/ml, and if you filled a syringe with this, then the syringe would contain around 95% oxygen and only 5% would be ozone.

      If you use lower ozone concentrations, then this number falls even more.

      At an ozone concentration of 15 mcg/ml you’d be using around 1 to 2% ozone and the rest, so 98 to 99% would be oxygen.

      So when using ozone therapy, you ALWAYS use a dilution.

      Using 100% ozone – this is impossible with any of the common home ozone generators, and even less so with professional medical machines.

      The dissolution of the latex glove – this will happen at ANY ozone concentration, even the lowest one.

      I hope this helps.


      • victoria

        I am deeply thankful to you, Paola!
        You are awesome!

      • Paola Dziwetzki

        Hi Victoria,

        thank you very much!


  3. Jacquelyn Fellows

    Paola you are the best! I started IM’s before you posted these directions and I was winging it. I definitely learned from your tutorial..thank you for being so clear about the procedure. The current flu economy has me so strapped but in a few months when I recover I am looking forward to a private session with you.

    • Paola Dziwetzki

      Hi Jacqui,

      happy to hear that it was helpful!

      Whenever you’re ready – you know where to find me :-). And thank you for your earlier donation!


  4. Joy

    Hello! How often do you think you can perform this therapy? How often should you switch up sides? Thank you!

    • Paola Dziwetzki

      Hi Joy,

      I would switch up sides every time.
      I think as long as there is bruising or pain or any other irritation, you can do it indefinitely.
      You can also use your thigh muscles.


      • Joy

        Thank you! What is the thigh muscle you can inject into? I have to YouTube it!

  5. Phill B

    Thanks for the info. Can the same procedure be used direct into ankles and knees?

    • Paola Dziwetzki

      Hi Phill,

      with a smaller needle and a slightly different technique, yes.


  6. Jimmy

    I always enjoy reading your articles. There always full of interesting and useful information. Thank you for posting and sharing this.

    • Paola Dziwetzki

      Hi Jimmy,

      thank you!

  7. Deidre Bridges

    You are simply the best. I have learned so much from you and can’t wait to try this. Thank you for sharing so much with us so that we can all experience better health! God bless you, Paola!

    • Paola Dziwetzki

      Hi Deirdre,

      thank you! I appreciate you, too!



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