How to Do Subcutaneous Ozone Knee Injections

by | Last updated: Aug 7, 2021 | 38 comments

Subcutaneous ozone knee injections are a simple and effective treatment for joint pain.

In the case of inflammation or any type of damage to the soft tissue of the knee area, ozone injections can offer an immediate and substantial relief. 

Subcutaneous knee injections are different from true joint or intra-articular injections (named “prolozone” injections by Dr. Frank Shallenberger) in that they are shallower and less ozone is used. 

The needle used in subcutaneous ozone injections is smaller and shorter than what is used for true intra-articular injections. 

For that reason they do not require anesthetics, are simpler to perform, and carry less risk than prolozone treatments. 

What equipment do you need for subcutaneous ozone knee injections?

Every ozone set-up consists of the following four parts:

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1. Oxygen source plus regulator, here it’s a  medical oxygen tank and with a matching low flow regulator (Learn about other options here). Don't forget the wrench for the tank. 

2. Ozone generator, here it’s a Promolife Dual

3. Accessories: silicone tubing (around 6 ft. / 1.5 m), syringe, needle: 30g (13mm long), alcohol wipes, cotton pads, bandaids

4. Breathing protection: Syringe filling station

If you decide to buy from Promolife, use discount code TPO7D21 to get 7% off on ozone gear.

How to do subcutaneous ozone knee injections?


Suggested settings

Ozone concentration: 10 to 30 mcg/ml
Oxygen flow: not important with a syringe, depends on ozone generator
Gas volume: 2 to 5 ml per puncture site, 5 to 10 ml per knee


Suggested settings

Ozone concentration:

10 to 30 mcg/ml

Oxygen flow:

not important with a syringe, depends on ozone generator

Gas volume:

2 to 5 ml per puncture site, 5 to 10 ml per knee

Learn how to protect yourself from breathing ozone

1. Screw the low flow regulator onto the oxygen tank.

2. Take a piece of silicone tubing, and connect one end to the oxygen tank. 

3. Take the other end of the silicone tubing and a luer lock connector and push one into the other.

4a luer lock and silicone tubing

4. Screw the silicone tubing with the luer lock into the “oxygen in” port of the ozone generator. 


5. Screw the syringe filling station onto the ozone outlet of the ozone generator. Make sure the OFF valve is turned towards the destructor. 



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6. Plug the ozone generator into the power supply. 


7. Open the valve of the oxygen tank, just half a turn. Watch the regulator and see how the gauge jumps up. It shows you how much oxygen is left in the tank. 

8. Open the regulator to 1/2 LPM. 


9. Flush the lines: Count to 15 to let pure oxygen flow through the lines, the syringe filling station, and the filter.


10. Flush the syringe: screw it onto the syringe port of the syringe filling station and fill with pure oxygen. 

10 flush syringe

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11. Unscrew and empty it. 

11 unscrew and empty it

12. Close the valve of the syringe filling station.


13. Screw the syringe onto the syringe port of the syringe filling station.

15 screw syringe onto filling station

14. Check your ozone output chart and see which settings you will need. Choose an ozone concentration between 10 and 30 mcg/ml. I pick 15 mcg/ml. Which means I will need to keep the oxygen flow at 1/2 LPM and the first ozone dial on 5, the second remains off. 

15. Set the dial on your ozone generator to the desired setting. In my example I set the first dial on 5, the second dial remains off. 



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16. Turn the lever on the syringe filling station to open the syringe port.


17. Fill the syringe with around 20 cc of ozone/oxygen mix if you will inject one knee. 


18. Close the lever on the syringe filling station when the syringe is 20 cc full.


19. Turn the ozone generator off. 


20. Close the regulator by setting it to zero. 

21 close regulator

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21. Unscrew the syringe. Keep it upright.

22. Unpack the needle. 


23. Screw the needle onto the syringe. 


24. Set is aside for a moment. 


25. Put gloves on



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26. Sit in a position so that your knees are at a 90 degrees angle.


27. Locate the spots where you will inject the ozone, see the two pink circles in the picture. You can inject each knee on both sides of the patella. The injection spots are located left and right underneath the patella, in between the femur and the tibia. When you palpate them, you will feel two soft dimples. 

knee-sketch-drawing 4

28. Disinfect the injection site with an alcohol wipe. 


29. Remove the cap from the needle. Flush it with ozone by pressing out a few ml. 


30. Inject straight into one of the soft spots. 


31. Press in a few ml of ozone/oxygen gas mix. You may feel a sensation of pressure. 


32. Pull the needle out. Press a cotton pad on the puncture wound to stop the bleeding. 


33. Repeat steps 30 to 32 on the other side of the same knee, and if necessary also on the other knee. 


34. Put bandaids on.


35. Dispose of the needle in the sharps bin. 


36. When you're done with ozone for the day, make sure to release the pressure from the regulator. First, close the valve on the oxygen tank. Do not exert too much force but make sure you close it tightly. 

36 close tank

37. Open the regulator all the way to the maximum flow. Watch the gauge on the regulator go down to zero within seconds. 

37 open all the way original

38. Once the gauge is at zero, set the regulator to zero. You're done!


What are subcutaneous ozone knee injections used for?

Subcutaneous ozone injections into the knee can offer relief for the following conditions: 

  • joint pain of any kind
  • arthritis
  • arthrose
  • bursitis
  • damage to the ligaments
  • damage to the cartilage
  • any type of inflammation in or around the joint

Risks and contraindications for subcutaneous ozone knee injections:


Bleeding, pain, inflammation and infection from the injection site


Allergy, possibly transplanted organs

The Crazy Ozone Lady's take on subcutaneous ozone knee injections

I am a great fan of subcutaneous ozone injections into the knees and other joints.

They are simple and easy to perform, are low risk, and can significantly reduce joint pain.

They are not as effective as true intra-articular joint injections.

Therefore, in order to achieve the same effect, multiple subcutaneous injections may be required.

The advantage of shallow ozone knee injections is that they do not call for an anesthetic, they are easy to learn, and they can be repeated as often as the need arises.

I’ve applied them on myself and many times on my Dad.

About the author:

PaolaI’m Paola the Crazy Old Ozone Lady behind The Power of Ozone. I’m a licensed naturopathic practitioner, natural health consultant, ozone therapy enthusiast, researcher, and ozone therapy analyst. I hold certificates in ozone therapy, hyperbaric ozone applications, Oxyvenierung, and the Andrew Cutler chelation. I own several ozone generators including a German hyperbaric 10 Pass machine. I have been using ozone for over 13 years, I’ve chelated with the ACC program for close to 5 years and I’ve been carnivore for nearly 1.5 years. This website serves as a resource for those who are interested in ozone therapy and other approaches to successfully manage chronic conditions.

Legal Disclaimer

Information provided is for informational purposes only and is not a substitute for professional medical advice. No health claims for these products or treatments have been evaluated by the United States Food and Drug Administration (FDA), nor has the FDA nor any other medical authority approved these treatments or products to diagnose, cure, or prevent disease. Neither The Power of Ozone nor the manufacturers of these items are responsible for the misuse of this equipment or the information on this page. It is highly advised to receive professional council from a licensed doctor before using ozone therapy or any of the mentioned products or tests on yourself.

Affiliate Disclosure

This website contains links to vendors of products I endorse, including If you decide to make a purchase through one of my links, they will pay me a commission. This does not influence my opinion about the products, as you can see from my reviews.


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Affiliate Disclosure

This website contains links to vendors of products I endorse, including If you decide to make a purchase through one of my links, they will pay me a commission. This does not influence my opinion about the products, as you can see from my reviews.


  1. Carol

    Thank you for this invaluable information! Do you think ozone knee injections can help with meniscus issues?

    • Paola Dziwetzki

      Yes, I do.

  2. Carla

    Is it necessary to use Medical Oxygen when doing these injections or would Industrial oxygen with a filter suffice?

    • Paola Dziwetzki

      Hi Carla,

      I would go with medical oxygen for any type of ozone injections …


  3. Chris

    Many thanks Paola, for a great website, you are a real trooper leading us to health and self sufficiency!

    I was wondering if you need to use a new needle every time when injecting the Knees at one session or one needle can be used for all 4 injections?

    Thanks again.

    • Paola Dziwetzki

      Hi Chris,

      you can use one needle for all four injections during one session.


  4. Jas

    Hi Paolo. I am finding your website very helpful. Thank you. I have osteoarthritis of the hip, and I have been exploring varying Ozone treatments to find relief, and to hopefully find a regenerative path. I have been doing rectal insufflations and drinking Ozone water, but I am seeking local intraarticular prolozone injections (with little success). This article has me considering subcutaneous injections around the hip. I will look online for injection points for the hip. I appreciate your injection point diagram for the knee (and your DIV guidance), and I hope to find something similar for my right hip. Any suggestions. Again, thank you for your voluminous detailed work. It's time for me to purchase your ebook.

    • Paola Dziwetzki

      Hi Jas,

      I hate to be a party pooper, but if intraarticular injections have not rendered much of a success, I doubt very much that subcutaneous ones would ….

      I don't have a similar diagram for hip injections, but since there is much more fat deposit around the hip (more so for women than men, generally), it should be less of a problem to inject around the hip (I can't tell from your name if you are male or female). You can also always pinch a layer of the skin/fat and inject in that fold.

      You may also want to look into a low vitamin A diet: this has resolved my joint pain. So muscle meat, beans and rice.


  5. Ann Jones

    1. I would like to use a filter on the needle for subcutaneous injections in case there are particulate matter from the oxygen tank or generator. What gauge would you recommend?

    2. Can cupping be used to treat facial wrinkles?

    • Paola Dziwetzki

      Hi Ann,

      1. so the filters don't have any specific gauge, but they come with different connectors. I recommend this luer lock filter:

      2. I don't think that this is a good method to treat wrinkles. But you can try. I did see some positive effects for a while with the O3Elite cream:


  6. Peter

    Hello to fellow Ozone users…..I want to tell you about ozone injections into the knee's, to begin with I want to say right away that using 30 ga. needles are completely pain free,and you don't even feel the needle going in…… My situation is that I have arthritis that attacks the joints and in particular my knee joints, so bad that at times tears would come or you get kind of sick to your stomach from the pain, I won't use drugs on my body so I was barely crawling around most of the time….Then I discovered Ozone thru Paula's site and then said I think I can do this, as her website with pictures told me everything I needed, I rounded up all of the equipment and bought the needles…… Now I was committed, you are always a little hesitant the first time, so I had a lady friend help to give the injections, it took almost no time, less then 5 min. for both knee's … Right after the injections, your knees; feel a little different, with less pain, but the real surprise was that the next morning I was completly pain free, you just can't believe what a major relief it is to be pain free… What Paula is doing is just short of miracle work, if I had not found her site I would still be in pain, but now I know I can fix myself and now you can too..

    • Paola Dziwetzki

      Hi Peter,

      thank you for sharing your story !!!


  7. Christy

    Hi Paola. Thank you for the excellent information about how to do different ozone treatments. I'd like to do subcutaneous ozone injections into a few joints in my hands and feet as I have RA (rheumatoid arthritis). Your tutorial was about injecting a large joint, the knee. Would you still recommend the same type of needle ( 30g, 13mm long) for the smaller joints in the hands and feet?

    • Paola Dziwetzki

      Hi Christy,

      For smaller joints I would first try ozone sauna, or soaking in water with hydrogen peroxide, or do an ozone bath, and change to a meat based, anti-inflammatory diet.

      If that didnt' work, I would possible try injections. And yes, you can use the same needles for that.


  8. Frank T

    How deep do you insert the needle for the knee injection? It appears from the photo that it's inserted as far as it will go, but that may be a feature of the photo. Would very much appreciate clarification on this. (and thanks for all your work and shared info about ozone!!!)

    • Paola Dziwetzki

      Hi Frank,

      Yes, you can go as far as the needle goes (13 mm).


  9. Norman

    Hi Paola,
    Can I follow the same protocol for shoulder Thanks

    • Paola Dziwetzki

      Hi Norman, yes you can.

  10. Nick Fedan

    Hi Paola,

    Your work is clear, concise, accurate, and experienced-based. Thank you.

    I have taken a couple of Dr.Shallenberger's courses, but (as you know) he stays away from DIV as practiced by Dr. Robins and endorsed by Dr. Rowen. I am interested in a consult (via Zoom?) with you specifically about DIV. Would appreciate your comments via my email.
    Cheers, Nick

    • Paola Dziwetzki

      Hi Nick,

      I sent you an email. Please check your inbox.


    • Teresa

      How do you use the EXT50 for injections?

    • Paola Dziwetzki

      Hi Teresa,

      I am not sure Nick saw your post. You can get an attachment for the EXT50 with a three way valve (typically yellow with Longevity products) that allows you to attach a syringe and so fill it. then you can do injections like described above.


  11. Franco

    Hi Paola,

    Can I use an O2 concentrator for injections vs. a medical O2 tank?

    Pro and cons and safety concerns you can share?


  12. Jacob

    Thank you Paola for all the info!
    I have had ligament issues in the wrist. Could give me a hint of how to inject in the area? Volumes and concentration?

    • Paola Dziwetzki

      Hi Jacob,

      First, I wouldn't do injections at all, but either limb bagging, ozone sauna, or soaking the wrist in hot water with 1/2 cup of 35% hydrogen peroxide.

      Applying DMSO could also do the trick.

      Injecting the wrist is a delicate matter and it's not easy to explain it through the comments section. So, if all of the above would not work out, I would probably take a very small needle, 30g 13mm or smaller and inject very shallow intradermally or subcutaneously. Set “ozone bubbles” to be absorbed by the tissue over time. But as mentioned, I would try easier things first.


    • Jacob

      Thanks for all advice, Paola.

      As curiosity, have you seen wrist instability (dislocation) been healed by any of these procedures be it intraarticular, subcutaneous , etc?

    • Paola Dziwetzki

      No, nothing that I recall ….

    • Jacob

      Is the claim that ozone and ozonides look for inflamed and problematic areas? In this case a small volume DIV to a big hand vein (like the ones I have) could possibly affect the wrist? Sounds crazy but we are on the Crazy Lady's website …

    • Paola Dziwetzki

      Hi Jacob,

      ozone therapy is an excellent anti-inflammatory treatment. So if the wrist problem is because of inflammation, DIVs could help.

      Alternatively, you could also do ozone saunas, or peroxide baths by submerging the hand in hot water with 1/4 cup of 35% peroxide.


    • Barry

      Anyone may write to president Trump about using Ozonated Water at

    • Vicki

      Where do you find needle tips to screw on the plastic syringe? Are they just regular 30 guage hyperdermic needle tips, and will they screw on, or do the syringe and needle come together. I am askig this because every plastic syringe I find with a needle attached is a blunt needle, saying it is for scientific purposes. Thank you. I enjoy your site so much, I just bought my generator package and have a lot of issues to treat.

  13. Dennis Anderson

    Thank you Paola. Your pictures and descriptions of the procedure are excellent! I am a retired dentist and still have all of my ozone equipment from my dental office, so I can try your procedure if I need it for my wife or myself. We are waiting things out in Thailand right now, but I always bring my ozone equipment with me wherever I go and use it for ri and ozonated water. I had an episode of ear pain after doing ei and appreciated your warning, but saw it a little late.
    Thanks for all your help!

    • Paola Dziwetzki

      Hi Dennis,

      I hope it will help you and your wife when you try it.

      Let me know how it will go!


    • Vicki

      Where do you find needle tips to screw on the plastic syringe? Are they just regular 30 guage hyperdermic needle tips, and will they screw on, or do the syringe and needle come together. I am askig this because every plastic syringe I find with a needle attached is a blunt needle, saying it is for scientific purposes. Thank you. I enjoy your site so much, I just bought my generator package and have a lot of issues to treat.

    • Paola Dziwetzki

      There are plenty of 30g needles on amazon, not blunt, but real needles.

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