How to Do Subcutaneous Ozone Knee Injections

by | Jun 21, 2020 | 4 comments

Subcutaneous ozone knee injections are a simple and effective treatment for joint pain.

In the case of inflammation or any type of damage to the soft tissue of the knee area, ozone injections can offer an immediate and substantial relief. 

Subcutaneous knee injections are different from true joint or intra-articular injections (named “prolozone” injections by Dr. Frank Shallenberger) in that they are shallower and less ozone is used. 

The needle used in subcutaneous ozone injections is smaller and shorter than what is used for true intra-articular injections. 

For that reason they do not require anesthetics, are simpler to perform, and carry less risk than prolozone treatments. 

What equipment do you need for subcutaneous ozone knee injections?

  • oxygen tank and regulator 
  • tank wrench
  • ozone generator
  • ozone syringe
  • syringe filling station with a filter
  • 30g 13 mm needle
  • alcohol wipes
  • cotton pad
  • bandaid
  • gloves
  • sharps bin

Scroll down to learn where you can order the recommended equipment and supplies.

How to do subcutaneous ozone knee injections?


Suggested settings

Ozone concentration:10 to 30 mcg/ml
Oxygen flow:not important with a syringe, depends on ozone generator
Gas volume:2 to 5 ml per puncture site, 5 to 10 ml per knee


Suggested settings

Ozone concentration:

10 to 30 mcg/ml

Oxygen flow:

not important with a syringe, depends on ozone generator

Gas volume:

2 to 5 ml per puncture site, 5 to 10 ml per knee

1. Screw the low flow regulator onto the oxygen tank.

2. Take a piece of silicone tubing, and connect one end to the oxygen tank. 

3. Take the other end of the silicone tubing and a luer lock connector and push one into the other.

4a luer lock and silicone tubing

4. Screw the silicone tubing with the luer lock into the “oxygen in” port of the ozone generator. 


5. Screw the syringe filling station onto the ozone outlet of the ozone generator. Make sure the OFF valve is turned towards the destructor. 


6. Plug the ozone generator into the power supply. 


7. Open the valve of the oxygen tank, just half a turn. Watch the regulator and see how the gauge jumps up. It shows you how much oxygen is left in the tank. 

8. Open the regulator to 1/2 LPM. 


9. Flush the lines: Count to 15 to let pure oxygen flow through the lines, the syringe filling station, and the filter.


10. Flush the syringe: screw it onto the syringe port of the syringe filling station and fill with pure oxygen. 

10 flush syringe

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11. Unscrew and empty it. 

11 unscrew and empty it

12. Close the valve of the syringe filling station.


13. Screw the syringe onto the syringe port of the syringe filling station.

15 screw syringe onto filling station

14. Check your ozone output chart and see which settings you will need. Choose an ozone concentration between 10 and 30 mcg/ml. I pick 15 mcg/ml. Which means I will need to keep the oxygen flow at 1/2 LPM and the first ozone dial on 5, the second remains off. 

15. Set the dial on your ozone generator to the desired setting. In my example I set the first dial on 5, the second dial remains off. 


16. Turn the lever on the syringe filling station to open the syringe port.


17. Fill the syringe with around 20 cc of ozone/oxygen mix if you will inject one knee. 


18. Close the lever on the syringe filling station when the syringe is 20 cc full.


19. Turn the ozone generator off. 


20. Close the regulator by setting it to zero. 

21 close regulator

21. Unscrew the syringe. Keep it upright.

22. Unpack the needle. 


23. Screw the needle onto the syringe. 


24. Set is aside for a moment. 


25. Put gloves on


26. Sit in a position so that your knees are at a 90 degrees angle.


27. Locate the spots where you will inject the ozone, see the two pink circles in the picture. You can inject each knee on both sides of the patella. The injection spots are located left and right underneath the patella, in between the femur and the tibia. When you palpate them, you will feel two soft dimples. 

knee-sketch-drawing 4

28. Disinfect the injection site with an alcohol wipe. 


29. Remove the cap from the needle. Flush it with ozone by pressing out a few ml. 


30. Inject straight into one of the soft spots. 


31. Press in a few ml of ozone/oxygen gas mix. You may feel a sensation of pressure. 


32. Pull the needle out. Press a cotton pad on the puncture wound to stop the bleeding. 


33. Repeat steps 30 to 32 on the other side of the same knee, and if necessary also on the other knee. 


34. Put bandaids on.


35. Dispose of the needle in the sharps bin. 


36. When you’re done with ozone for the day, make sure to release the pressure from the regulator. First, close the valve on the oxygen tank. Do not exert too much force but make sure you close it tightly. 

36 close tank

37. Open the regulator all the way to the maximum flow. Watch the gauge on the regulator go down to zero within seconds. 

37 open all the way original

38. Once the gauge is at zero, set the regulator to zero. You’re done!


Recommended equipment for subcutaneous ozone knee injections

This is the equipment I used in the pictures above:

If you decide to buy from Promolife, use the following discount code to get 7% on ozone gear: TPO7D.

What are subcutaneous ozone knee injections used for?

Subcutaneous ozone injections into the knee can offer relief for the following conditions: 

  • joint pain of any kind
  • arthritis
  • arthrose
  • bursitis
  • damage to the ligaments
  • damage to the cartilage
  • any type of inflammation in or around the joint

Risks and contraindications for subcutaneous ozone knee injections:


Bleeding, pain, inflammation and infection from the injection site


Allergy, possibly transplanted organs

Success stories with subcutaneous ozone knee injections

SUBQ-O3-knee-pain 1


The Crazy Ozone Lady’s take on subcutaneous ozone knee injections

I am a great fan of subcutaneous ozone injections into the knees and other joints.

They are simple and easy to perform, are low risk, and can significantly reduce joint pain. 

They are not as effective as true intra-articular joint injections.

Therefore, in order to achieve the same effect, multiple subcutaneous injections may be required. 

The advantage of shallow ozone knee injections is that they do not call for an anesthetic, they are easy to learn, and they can be repeated as often as the need arises.

I’ve applied them on myself and many times on my Dad. 

Legal Disclaimer

Information provided is for informational purposes only and is not a substitute for professional medical advice. No health claims for these products or treatments have been evaluated by the United States Food and Drug Administration (FDA), nor has the FDA nor any other medical authority approved these treatments or products to diagnose, cure, or prevent disease. Neither The Power of Ozone nor the manufacturers of these items are responsible for the misuse of this equipment or the information on this page. It is highly advised to receive professional council from a licensed doctor before using ozone therapy or any of the mentioned products or tests on yourself.

About the author:

PaolaI’m Paola the Crazy Old Ozone Lady behind The Power of Ozone. I’m a licensed naturopathic practitioner, natural health consultant, ozone therapy enthusiast, researcher, and ozone therapy analyst. I hold certificates in ozone therapy, hyperbaric ozone applications, Oxyvenierung, and the Andrew Cutler chelation. I own several ozone generators including a German hyperbaric 10 Pass machine. I have been using ozone for over 13 years, I’ve chelated with the ACC program for close to 5 years and I’ve been carnivore for nearly 1.5 years. This website serves as a resource for those who are interested in ozone therapy and other approaches to successfully manage chronic conditions.

Paola Dziwetzki, NP

I’m Paola, aka the Crazy Old Ozone Lady. I help people use ozone therapy in the most effective way. I have used ozone and diet to successfully treat psoriasis, joint pain, chronic fatigue, a low immune system, herpes infections, and a few other conditions.
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Affiliate Disclosure

This website contains links to vendors of products I endorse, including If you decide to make a purchase through one of my links, they will pay me a commission. This does not influence my opinion about the products, as you can see from my reviews.


  1. Dennis Anderson

    Thank you Paola. Your pictures and descriptions of the procedure are excellent! I am a retired dentist and still have all of my ozone equipment from my dental office, so I can try your procedure if I need it for my wife or myself. We are waiting things out in Thailand right now, but I always bring my ozone equipment with me wherever I go and use it for ri and ozonated water. I had an episode of ear pain after doing ei and appreciated your warning, but saw it a little late.
    Thanks for all your help!

    • Paola Dziwetzki

      Hi Dennis,

      I hope it will help you and your wife when you try it.

      Let me know how it will go!


    • Barry

      Anyone may write to president Trump about using Ozonated Water at


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