How to Do Sublingual Ozone Treatments?

Sublingual ozone treatments are a new form of using ozone therapeutically.
During this procedure ozone gas is placed under the tongue, so it’s a form of mouth insufflation with ozone.
The recommended ozone concentration ranges between 20 and 25mg/ml and the suggested ozone amounts between 20ml and 40 ml.
During the whole time the tongue is firmly pressed against the palate and breathing takes place only through the nose. This prevents the accidental breathing of ozone gas during sublingual ozone treatments.
The goal is to use the ability of the sublingual area to absorb medication, in this case ozone, and to achieve a systemic effect.
What equipment do you need for sublingual ozone treatments?
- an oxygen source (here an oxygen tank and regulator)
- ozone generator
- syringe filling station
- ozone resistant syringe
- 2 inch piece of silicone tubing
- luer lock connectors
Scroll down to find a list with the equipment you see in the pictures below.
Suggested settings
Ozone concentration: | 20 to 25 mcg/ml |
Oxygen flow: | not important, since a syringe is used |
Gas volume: | 20 to max 40 ml |
Suggested settings
Ozone concentration:
20 to 25 mcg/ml
Oxygen flow:
not important with a syringe
Gas volume:
20 to max 40 ml
1. Screw a low flow regulator onto the oxygen tank.

2. Take a piece of silicone tubing, and connect one end to the oxygen tank.

3. Take a male luer lock connector and push it into the other end of the silicone tubing.

4. Screw the luer lock end into the “oxygen in” port of the ozone generator.

5. Attach a syringe filling station onto the ozone out port of the generator. Make sure that the off valve is turned towards the syringe port.

6. Assemble the mouth adapter: take a piece of silicone tubing, around four inches long and a female luer lock connector and push one into the other.

7. Set it aside for a moment.

8. Make sure the ozone generator is plugged into the power supply.

9. Open the valve of the oxygen tank. Half a turn is enough.

10. Check the ozone output chart for the desired settings. Here, I pick an ozone concentration of 19 mcg/ml at an oxygen flow of 1/2 LPM, which means I will set the first dial on 5 and the second on 1.

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11. Open the regulator to a flow of 1/2 LPM.

12. Turn on the ozone generator.

13. Let it run for 10 seconds.

14. Attach syringe onto syringe port.

15. Open the syringe port by turning the “off” valve on the syringe filling station towards the destructor.

16. Fill the syringe.

17. Once the syringe is full with around 20 cc of gas, close the syringe port.

18. Turn the ozone generator off.

19. Close the oxygen regulator, set it to 0.

20. Unscrew the syringe.

21. Attach the mouth adapter (step 6) to the syringe.

22. Insert the mouth piece under your tongue.

23. Close your mouth and keep the tongue pressed against your palate. Press 1 to 2 cc of gas under your tongue. Breathe only through your nose.

24. Wait 20 to 30 seconds. Let the ozone absorb. Keep breathing through your nose.

25. If too much saliva accumulates in your mouth, remove the mouth piece, exhale the gas and either spit out or swallow the saliva, and restart from step 22. Otherwise, skip this step and continue with 26.

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26. Repeat steps 23 to 25 until you empty the syringe. Keep breathing through your nose the whole time.

27. Once you have emptied the syringe, exhale the gas through your mouth one last time, and either swallow or spit out the saliva.

28. When you are done, make sure to remove pressure from the regulator. First, close the valve on the tank without using excessive force.

29. Open the regulator to the highest flow and watch the gauge go down.

30. As a last step set the regulator to zero. You're done!

Recommended equipment for sublingual ozone treatments
This is the equipment I used in the pictures above:
- industrial oxygen tank. (Learn more about the best options to get oxygen tanks)
- industrial low flow regulator, CGA540
- Promolife Dual Cell ozone generator
- syringe filling station
- ozone resistant syringe
- extra silicone tubing
- luer lock connector
Promolife offers a 7% discount on most ozone equipment if you use the following code: TPO7D.
Risks of and contraindications for sublingual ozone treatments
Risks:
- accidentally breathing in ozone
Contraindications:
- inability to breathe through the nose with a closed mouth (for example due to sinus congestion)
- acute asthma attack
- transplanted organs, since ozone therapy could overstimulate the immune system and trigger a rejection of foreign organs
The Crazy Ozone Lady's take on sublingual ozone treatments
For the purpose of this guide I've done a few of the sublingual ozone treatments, and here is my opinion about them:
I'm not a fan.
I doubt I will ever repeat them again. In spite of taking great care to keep the tongue pressed against my palate, each time I did the insufflation, I ended up accidentally breathing some ozone.
In spite of keeping my tongue pressed against the palate and in spite of keeping my mouth firmly closed, some ozone managed to escape and I ended up inhaling it through the nose.
Success stories with sublingual ozone treatments
A medical professional in The Ozone Group reported that a patient with trigeminal neuralgia felt an immediate improvement after sublingual ozone applications combined with other treatments.
Don't just play around with ozone treatments. Go in all the way to get the best results. Let me show you how.
About the author:
I’m Paola the Crazy Old Ozone Lady behind The Power of Ozone. I’m a licensed naturopathic practitioner, natural health consultant, ozone therapy enthusiast, researcher, and ozone therapy analyst. I hold certificates in ozone therapy, hyperbaric ozone applications, Oxyvenierung, and the Andrew Cutler chelation. I own several ozone generators including a German hyperbaric 10 Pass machine. I have been using ozone for over 13 years, I’ve chelated with the ACC program for close to 5 years and I’ve been carnivore for nearly 1.5 years. This website serves as a resource for those who are interested in ozone therapy and other approaches to successfully manage chronic conditions.
Legal Disclaimer
Information provided is for informational purposes only and is not a substitute for professional medical advice. No health claims for these products or treatments have been evaluated by the United States Food and Drug Administration (FDA), nor has the FDA nor any other medical authority approved these treatments or products to diagnose, cure, or prevent disease. Neither The Power of Ozone nor the manufacturers of these items are responsible for the misuse of this equipment or the information on this page. It is highly advised to receive professional council from a licensed doctor before using ozone therapy or any of the mentioned products or tests on yourself.
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